Item #:
125660636
20x Tris-Acetate-SDS Running Buffer (500 ML)
93% of respondents would recommend this to a friend
Item #:
125660636
CDF 216660
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Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer.
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What Stands Out
High Purity
This running buffer is formulated with high-purity reagents, ensuring reliable and reproducible results in electrophoresis applications, making it ideal for both research laboratories and educational institutions.
Convenience Pack
The convenient 500 ML packaging allows for easy handling and storage, providing sufficient volume for multiple experiments while maintaining product stability and prolonging shelf life.
Versatile Application
Designed for Tris-Acetate-SDS systems, this running buffer supports a range of protein separation techniques, catering to diverse scientific needs and enhancing productivity in both small-scale and large-scale experiments.
Product Details
| Brand Name | Generic |
| Model Number | TAS01-500ML |
| Number of Items | 1 |
| Manufacturer | Bioland Scientific LLC |
| Manufacturer Part Number | TAS01-500ML |
| Brand | Generic |
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists conducting SDS-PAGE experiments to analyze protein samples effectively.
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Educational Institutions
Well-suited for labs and classrooms focusing on protein separation techniques in educational settings.
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Protein Research
Beneficial for researchers studying protein interactions, structures, and molecular weight using electrophoresis.
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Casual Hobbyists
Not suitable for casual users without proper lab equipment and knowledge of protein analysis.
Product Description
20x Tris-Acetate-SDS Running Buffer (500 ML)
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Customer Questions & Answers
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Question:
What is 20x Tris-Acetate-SDS Running Buffer used for?
Answer: 20x Tris-Acetate-SDS Running Buffer is primarily used for protein electrophoresis in molecular biology labs. It facilitates the separation of proteins based on their size during gel electrophoresis. The buffer's high ionic strength and unique composition help stabilize pH levels during the run, ensuring more precise and reproducible results. Researchers often utilize this buffer when analyzing protein samples, confirming protein purity, or assessing molecular weight for better understanding of protein behavior in processes like Western blotting. -
Question:
How do you prepare the 1x working solution from 20x Tris-Acetate-SDS Running Buffer?
Answer: To prepare a 1x working solution from a 20x Tris-Acetate-SDS Running Buffer, simply dilute 1 part of the 20x buffer with 19 parts of distilled water. For example, if you need 500 mL of 1x solution, mix 25 mL of the 20x buffer with 475 mL of distilled water. This simple dilution effectively adjusts the buffer concentration for optimal performance in gel electrophoresis, helping to ensure precise protein separation and enhanced analysis. -
Question:
What types of samples can be run using this buffer?
Answer: This buffer is ideal for a variety of protein samples such as purified proteins, cell lysates, or recombinant proteins. It is commonly used for analyzing proteins extracted from biological tissues, microorganisms, or cultured cells. By ensuring consistent pH stability and optimal ionic strength, it enables accurate size-based separation of various protein types during SDS-PAGE. This versatility makes it a staple in laboratories focusing on protein research, diagnostics, or biotechnology applications. -
Question:
Is 20x Tris-Acetate-SDS Running Buffer suitable for Western blotting?
Answer: Yes, 20x Tris-Acetate-SDS Running Buffer is suitable for Western blotting applications. After proteins are separated by SDS-PAGE using this buffer, they can be transferred to a membrane for further analysis. The buffer's composition promotes effective protein migration and maintaining structural integrity, which is crucial for accurate detection later in the Western blotting process. Many researchers depend on it for reliable results in protein identification and quantification. -
Question:
What storage conditions are recommended for this buffer?
Answer: 20x Tris-Acetate-SDS Running Buffer should be stored at room temperature, away from direct sunlight and extreme temperature fluctuations. Proper storage helps maintain its chemical stability and effectiveness for electrophoresis. Additionally, always ensure that the bottle is tightly sealed to prevent contamination or evaporation of essential components. Adequate storage practices extend the buffer’s usability, making it ready for your experiments whenever needed. -
Question:
What is the pH range of the 1x working solution?
Answer: The pH of the 1x working solution of 20x Tris-Acetate-SDS Running Buffer typically ranges between 7.2 and 8.4, depending on the specific formulation. This pH range is critical for maintaining the integrity of proteins during electrophoresis while ensuring optimal separation conditions. Many users find that this pH range supports a wide array of protein types, enhancing the reproducibility of their experimental outcomes. -
Question:
Can this buffer be used for DNA electrophoresis as well?
Answer: No, 20x Tris-Acetate-SDS Running Buffer is specifically designed for protein electrophoresis. The presence of SDS in the buffer denatures protein structures, allowing them to be separated based on size. For DNA electrophoresis, a different buffer, such as TAE or TBE, is typically used as they do not denature nucleic acids and provide appropriate charge and stability for DNA fragments. It’s essential to choose the right buffer depending on your analysis needs. -
Question:
What equipment do I need to use this running buffer?
Answer: To utilize 20x Tris-Acetate-SDS Running Buffer, you'll need basic equipment such as an electrophoresis unit, power supply, and gel casting trays. Additionally, gel electrophoresis apparatus like combs and measuring devices are advisable for precise setup. This equipment works in tandem to ensure an effective separation process. Proper preparation and setup using the right tools enhance the efficiency and accuracy of your protein electrophoresis experiments. -
Question:
What are the safety precautions when using this buffer?
Answer: When using 20x Tris-Acetate-SDS Running Buffer, it's important to wear appropriate personal protective equipment (PPE) such as gloves, lab coats, and safety goggles. The buffer contains SDS, which can be irritating to the skin and eyes. Therefore, avoid direct contact, and always work in a well-ventilated area. Additionally, ensure proper lab protocols are followed for disposal of any waste or spillage to maintain a safe working environment. -
Question:
Where can I buy 20x Tris-Acetate-SDS Running Buffer 500 ML in Republic of the Congo?
Answer: You can buy 20x Tris-Acetate-SDS Running Buffer 500 ML on Ubuy. Ubuy offers a wide selection of laboratory supplies, including running buffers, enabling you to find the right products for your scientific needs. Their platform also provides important details about the product specifications and usage to assist you in making an informed purchase.
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CDF 216660
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Features & Benefits
- Highly effective for protein separation in denatured states.
- Optimized for large molecular weight proteins.
- Can be used with both Tris-Acetate SDS and LDS Sample Buffers.
- Compatible with Tris-Glycine Native Running Buffer for more effective native protein resolution.
- Stable for one year when stored at 4°C.
- Enhances the performance of Tris-Acetate gels significantly.
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