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Item #:
229814426
EGTA 0.5M, pH 8.0, 250mL
Item #:
229814426
CDF 259159
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EGTA is the most widely used calcium-selective chelator, 100,000 times more stable than its Mg complex.
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What Stands Out
High Purity
Contains high-purity EGTA, ensuring minimal contaminants for reliable experimental results, making it ideal for sensitive biochemical applications.
Precise pH Control
Formulated at pH 8.0 to maintain optimal conditions for enzymatic reactions, providing consistency and reproducibility in your research.
Convenient Packaging
Supplied in a 250mL bottle for easy handling and storage, allows for immediate use without the need for further preparation.
Product Details
| Country of Origin | This item will be imported from US |
| What is in the box | EGTA 0.5M, pH... For more details, please check description/product details |
Who Should Buy?
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Biochemists
Ideal for biochemists studying calcium-sensitive enzymes and protein interactions in controlled pH environments.
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Molecular Biologists
Essential for molecular biology experiments involving gene cloning and manipulation requiring calcium chelation.
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Laboratory Researchers
Useful for researchers conducting experiments that require consistent pH and calcium ion suppression.
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Non-Laboratory Use
Not suitable for casual or non-scientific applications due to specificity and required handling expertise.
Product Description
EGTA 0.5M, pH 8.0, 250mL
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is EGTA and its primary use in labs?
Answer: EGTA (ethylene glycol tetraacetic acid) is a chelating agent widely used in laboratories to bind calcium ions. This property makes it essential in biochemical experiments where the regulation of calcium levels is crucial. For instance, EGTA helps stabilize reactions that are sensitive to calcium, ensuring that enzyme activities are measured accurately in cell studies or buffered solutions. -
Question:
What is the significance of the pH level in EGTA solutions?
Answer: The pH level of a solution plays a vital role in its effectiveness as a chelator. A pH of 8.0 in EGTA solutions allows for optimal binding of calcium ions without significant interference from other metal ions, enhancing the precision of experiments. This controlled pH is particularly beneficial in biological assays where slight variations can lead to different outcomes. -
Question:
Can EGTA be used in cell culture applications?
Answer: Yes, EGTA can be used in cell culture applications, especially when calcium ion levels need to be controlled. For example, in studies involving cell signaling or apoptosis, EGTA is employed to manipulate calcium concentrations, aiding researchers in observing cellular behaviors without interference from calcium fluctuations. -
Question:
Is EGTA safe to handle in a laboratory environment?
Answer: While EGTA is commonly used in laboratories, it is essential to follow proper safety protocols. It is generally safe when used in recommended concentrations, but protective gear such as gloves and safety goggles should always be worn. Precautions are particularly important to avoid skin irritation or inhalation of dust during handling. -
Question:
How should EGTA be stored for optimal stability?
Answer: To maintain the stability of EGTA, it should be stored in a cool, dark place away from direct sunlight. Ideally, it should be kept in tightly sealed containers to prevent contamination and degradation. Proper storage ensures that the chemical retains its efficacy for various applications in biochemical and molecular biology research. -
Question:
What are some typical laboratory applications of EGTA?
Answer: EGTA is utilized in a variety of laboratory applications, including enzyme assays, protein purification, and cell biology experiments. For example, it is commonly used in protocols for investigating calcium-dependent processes in cells or to deactivate calcium-dependent enzymes, ensuring that experimental conditions remain consistent and controlled. -
Question:
Can EGTA be used in conjunction with other chelating agents?
Answer: Yes, EGTA can be effectively used alongside other chelating agents, such as EDTA, depending on the specific requirements of an experiment. This combination can provide a broader range of ion binding, allowing for more nuanced control over assay conditions. However, compatibility should be assessed based on the specific metals and pH needed in each scenario. -
Question:
Why is the concentration of EGTA important in experiments?
Answer: The concentration of EGTA is crucial because it determines the extent to which calcium ions can be bound. A concentration like 0.5M is effective for binding calcium effectively without overly complicating reaction conditions. Choosing the right concentration ensures that the experimental results are both accurate and reproducible across various setups. -
Question:
How does EGTA interact with calcium ions in a solution?
Answer: EGTA interacts with calcium ions through multiple coordination sites, effectively sequestering them in a stable complex. This process reduces the free calcium concentration in solutions that require tight regulation, which is especially important for studies focused on calcium-mediated cellular processes. This mechanism is foundational for maintaining cellular integrity during experimentation. -
Question:
Where can I buy EGTA 0.5M, pH 8.0, 250mL in Republic of the Congo?
Answer: You can purchase EGTA 0.5M, pH 8.0, 250mL from Ubuy, a reliable e-commerce platform that stocks a variety of laboratory chemicals and supplies. Their user-friendly interface makes it convenient for you to find and acquire this specific product for your research and experimental needs.
eCon Lab Supply Buffers Editorial Review
EGTA 0.5M, pH 8.0, 250mL is an essential reagent provided by eCon Lab Supply, designed for reliable laboratory applications. This product, weighing 4 ounces, comes in a well-sealed bottle to ensure quality and ease of use. It’s important to note that EGTA functions as a chelating agent, which is often utilized in various biochemical assays. Users appreciate its stable pH level, making it ideal for sensitive experiments. However, familiarity with lab protocols is advisable when working with such reagents to ensure safety and accuracy in results.
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Pros
- High-quality reagent for lab applications
- Stable pH for reliable results
- Convenient bottle for storage
- Lightweight, easy to handle
- Trusted brand in laboratory supplies
Cons
- May require careful handling in the lab
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CDF 259159
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Features & Benefits
- EGTA is a leading calcium-selective chelator.
- It forms a highly stable calcium complex.
- 100,000 times more stable than magnesium complexes.
- Ideal for preparing calcium buffers.
- Manufactured in a certified facility in the USA.
- Strict quality control and Good Manufacturing Practices are followed.
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